Ashbourne College offers a broad range of GCSE subjects.
| GCSE core subjects | Exam Board |
| English Language | EDEXCEL |
| English Literature | EDEXCEL |
| Mathematics | EDEXCEL |
| Science Double Awards | EDEXCEL |
| GCSE option subjects | Exam Board |
| Arabic* | EDEXCEL |
| Art & Design | AQA |
| Computer Science | Cambridge International |
| Drama | AQA |
| Economics | OCR |
| French | EDEXCEL |
| Geography | AQA |
| German | EDEXCEL |
| History | AQA |
| Italian * | EDEXCEL |
| Russian* | EDEXCEL |
| Spanish | EDEXCEL |
* Examination only (no tuition offered)